.. _cli::p3-submit-genome-assembly: ######################### p3-submit-genome-assembly ######################### *********************************** Submit a PATRIC Genome Assembly Job *********************************** .. code-block:: perl p3-submit-genome-assembly [options] output-path output-name Submit a set of one or more read libraries to the PATRIC genome assembly service. ************** Usage synopsis ************** .. code-block:: perl p3-submit-genome-assembly [-h] output-path output-name Submit an assembly job with output written to output-path and named output-name. The following options describe the inputs to the assembly: --workspace-path-prefix STR Prefix for workspace pathnames as given to library parameters. --workspace-upload-path STR If local pathnames are given as library parameters, upload the files to this directory in the workspace. --overwrite If a file to be uploaded already exists in the workspace, overwrite it on upload. Otherwise we will not continue the service submission. --paired-end-lib P1 P2 A paired end read library. May be repeated. --interleaved-lib LIB An interleaved paired end read library. May be repeated. --single-end-lib LIB A single end read library. May be repeated. --srr-id STR Sequence Read Archive Run ID. May be repeated. The following options describe the processing requested: --recipe Assembly recipe. Defaults to auto. Valid values are auto, unicycler, canu, spades, meta-spades, plasmid-spades, single-cell --trim-reads Trim reads before assembly --racon-iter Number of racon polishing iterations (for long reads) --pilon-iter Number of pilon polishing iterations (for short reads) --min-contig-len Filter out short contigs in the final assembly. --min-contig-cov Filter out contigs with low read depth in the final assembly. --genome-size Estimated genome size (for canu) The following options describe the read libraries: --platform STR The sequencing platform for the next read library or libraries. Valid values are infer, illumina, pacbio, nanopore, iontorrent --read-orientation-inward The reads in the next read library face inward (defaults to true) --read-orientation-outward The reads in the next read library face outward (defaults to false)