.. _cli::p3-submit-metagenomic-read-mapping: ################################## p3-submit-metagenomic-read-mapping ################################## ************************************* Submit a Metagenomic Read Mapping Job ************************************* This script submits a Metagenomic Read-Mapping job to BV-BRC. It takes input from read libraries and uses either the CARD or VFDB database to identify the nature of the incoming reads. ************** Usage Synopsis ************** .. code-block:: perl p3-submit-metagenomic-read-mapping [options] output-path output-name Start a metagenomic read mapping, producing output in the specified workspace path, using the specified name for the base filename of the output files. Command-Line Options ==================== - --paired-end-lib Two paired-end libraries containing reads. These are coded with a single invocation, e.g. \ ``--paired-end-lib left.fa right.fa``\ . The libraries must be paired FASTQ files. A prefix of \ ``ws:``\ indicates a file is in the BV-BRC workspace; otherwise they are uploaded from the local file system. This parameter may be specified multiple times. - --single-end-lib A library of single reads. This must be a FASTQ file. A prefix of \ ``ws:``\ indicates a file is in the BV-BRC workspace; otherwise they are uploaded from the local file system. This parameter may be specified multiple times. - --srr-id A run ID from the NCBI sequence read archive. The run will be downloaded from the NCBI for processing. This parameter may be specified multiple times. - --gene-set-name The gene set name-- \ ``CARD``\ or \ ``VFDB``\ . The default is \ ``CARD``\ . - --workspace-path-prefix Base workspace directory for relative workspace paths. - --workspace-upload-path Name of workspace directory to which local files should be uplaoded. - --overwrite If a file to be uploaded already exists and this parameter is specified, it will be overwritten; otherwise, the script will error out. - --help Display the command-line usage and exit. - --dry-run Display the JSON submission string and exit without invoking the service or uploading files.